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Ange values.Purification of Tric1SAMThe gene fragment corresponding to the

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Ange values.Purification of Tric1SAMThe gene fragment corresponding to the SAM domain of At3g49560/Tric1 (amino acids 191?61) was cloned in the pET15b vector (Novagen), yielding an N-terminal His tag fusion protein. Mutations corresponding to the Tric1SAM variants K15A and K20A were introduced by site-directed mutagenesis using the Quikchange II-XL kit (Agilent Technologies). The expression vector

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